K: koniocellular M: magnocellular P: parvocellular a: anterior p: posterior. ( B) Distribution of dissection ROI per donor within the MP cluster. ( A) Bar plot representing fraction of cells derived from a dissection region of interest (ROI) per cluster. ( F, G) UMAP representation, as in panel ( G), showing expression of the P marker FOXP2 (F) or the M marker ROBO2 in the macaque and human MP clusters ( G). The macaque nuclei are labeled based on cluster identity defined by the macaque snRNA-seq clusteranalysis, whereas the human nuclei within the MP cluster are labeled by dissection ROI.
( E) UMAP representation of joint analysis of human MP and macaque M and P nuclei using canonical correlation analysis (CCA). Select marker genes that are differentially expressed (p adj 1) are highlighted. ( D) 32 single-nucleus marker genes show consistent enrichment between M and P dissections in three donors.
( C) UMAP representation of neurons within the MP cluster, colored by donor. ( B) UMAP representation of human dLGN neurons colored by cluster. Bottom: gene expression dot plot showing the relative expression of marker genes (y-axis) across all clusters (x-axis). Numbers of nuclei in each cluster are indicated at the bottom of the dendrogram. Known marker genes were used for cluster identity assignment.
( A) Top: Cell-type taxonomy based on median cluster gene expression of 2000 differentially expressed genes and 946 nuclei from three donors.
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